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Sramkoski et al., In Vitro Cell Dev Biol Anim, 35: 5 [PMID: 10462204] CABRI:DSMZ_MUTZ Accession_number ACC 438 Cell_line_name 22RV1 Brief_description confirmed as human by cytogenetics cell line was derived from a human prostate carcinoma xenograft (CWR22R) that was serially propagated in nude mice after castration-induced regression and relapse of the parental, androgen-dependent CWR22 xenograft Description human prostate carcinoma cell line was derived from a human prostate carcinoma xenograft (CWR22R) that was serially propagated in nude mice after castration- induced regression and relapse of the parental, androgen-dependent CWR22 xenograft confirmed as human by cytogenetics Depositor Dr. J. W. Jacobberger, Case Western Reserve University, Cleveland, Ohio, USA Morphology epitheloid cells growing adherently in monolayers Culture_conditions 40% RPMI 1640 + 40% Dulbecco's MEM + 20% FBS split confluent culture 1:3 to 1:6 every 3-6 days using trypsin/EDTA; seed out at ca. 2-5 x 104 cells/cm 2 ; maximum density of ca. 5 x 105 cells/cm 2 at 37C with 5% CO2 cell harvest of ca. 4 x 105 cells/cm 2 Viruses PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV - Properties cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, GFAP -, neurofilament -, vimentin + Release_conditions - Hazard - Storage frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule Doubling_time ca. 40-60 hours Mycoplasma negative in DAPI, microbiological culture, RNA hybridization, PCR assays Fingerprint multiplex PCR of minisatellite markers revealed a unique DNA profile Cytogenetics human hyperdiploid karyotype with 8% polyploidy - 50(48-52)<2n>XY, +1, +7, +8, +12, i(1q), der(2)t(2;4)(p1?4;q3?3)del(2)(q11q3?1), der(4)t(2;4)(p1?4;q3?3), add(6)(q15), add(14)(q24) - sideline with del(1)(q21), +3, +18 - matches published karyotype Bibliographic_reference Wainstein et al., Cancer Res, 54: 5 (1994) [PMID: 7525052 ]

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